Marmara Pharmaceutical Journal 2017 , Vol 21 , Issue 4
Phytochemical composition and antifeedant activity of five Vincetoxicum taxa against Spodoptera littoralis and Leptinotarsa decemlineata
Sevda GÜZEL1,Roman PAVELA2,Ahmet İLÇİM3,Gamze KÖKDİL1
1Department of Pharmacognosy, Faculty of Pharmacy, Mersin University, 33169 Mersin, Turkey
2Crop Research Institute, Prague 161 06, Ruzyne, Czech Republic
3Department of Biology, Faculty of Arts and Science, Mustafa Kemal University, 31000 Hatay, Turkey
DOI : 10.12991/mpj.2017.28 Five Vincetoxicum taxa (V. canescens subsp. canescens, V. canescens subsp. pedunculata, V. fuscatum subsp. fuscatum, V. fuscatum subsp. boissieri and V. parviflorum) were investigated for insect antifeedant activity against Spodoptera littoralis and Leptinotarsa decemlineata. At a dose of 500 μg/cm2, 12 extracts were 100 % feeding deterrent, while 6 extracts showed 86.3- 99.3 % antifeedant activity. The effective doses (ED50) of the total ethanol extract from V. canescens subsp. pedunculata and of the methanol: dichloromethane (1:1) extract from V. parviflorum were 12, and 18 μg/cm2, respectively, against S. littoralis, and that of the total ethanol extract from V. fuscatum subsp. fuscatum was 25 μg/cm2 against L. decemlineata. The dichloromethane extracts of V. parviflorum and V. canescens subsp. pedunculata inhibited the growth of S. littoralis with ED50 values of 0.08 and 0.09 mg/g, respectively, and their LD50 values for larval mortality were 1.07 and 1.03 mg/g, respectively. Phytochemical analysis revealed the presence of cardiotonic glycosides, sugars, flavonoids and alkaloids in all investigated taxa. In the dichloromethane and methanol:dichloromethane (1:1) extracts, phenanthroindolizidine alkaloids were detected by LC/MS/MS. Keywords : Vincetoxicum; antifeedant activity; phytochemical composition; Spodoptera littoralis; Leptinotarsa decemlineata