Marmara Pharmaceutical Journal 2017 , Vol 21 , Issue 3
In vivo evaluation of Berberis vulgaris extract on acute toxoplasmosis in mice
Hossein Mahmoudvand1,Amir Tavakoli Kareshk2,Amir Keyhani2,Naser Zia-Ali2,Mohammad Reza Aflatoonian3
1Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
2Research Center for Tropical and Infectious Diseases, Kerman University of Medical Science, Kerman, Iran
3Bam University of Medical Sciences, Bam, Iran
DOI : 10.12991/marupj.319220 Since last centuries, herbal medicines have been considered as the main source for prevention and treatment a broad spectrum of illnesses as well as infectious diseases. Here we evaluated the in vivo activity of Berberis vulgaris methanolic root extract against acute toxoplasmosis induced by Toxoplasma gondii, RH strain. Male NMRI mice orally treated with B. vulgaris methanolic extract at the dose of 1 and 2 g/kg once a day for 14 days after 24 h mice were infected intraperitoneally with 104 tachyzoites of T. gondii, RH strain. The mortality rate in all infected mice and also parasite load were determined to assess the efficacy B. vulgaris methanolic extract against acute toxoplasmosis in mice model. The obtained results demonstrated that the mortality rate of infected mice was 100%, in 9th day after orally administration of B. vulgaris at the concentrations of 1 and 2 g/kg; while all mice in the control group were death in 5th day. Moreover obtained findings revealed that the mean number of tachyzoites was 131×104 and 79×104 for infected mice treated with B. vulgaris at the concentrations of 1 and 2 g/kg, respectively, whereas in control group the mean number of tachyzoites was 288×104 parasite. The obtained findings demonstrated the potential of B. vulgaris as a natural source for the production of new prophylactic agent for use in toxoplasmosis. Keywords : Toxoplasma gondii; Berberis vulgaris; Prophylactic; In vivo